VarTrace™: qPCR, Sanger

Features

Ultrasensitive

Limit of detection down to 0.1% VAF

Comprehensive

Detects up to 500 COSMIC mutations

Quantitative

Accurate quantitation

Sample Flexibility

Compatible with several sample types

Sample Data

NuProbe’s BDA technology enriches the variant allele frequency (VAF) of mutations by over 200-fold, improving Sanger’s limit of detection of 20% VAF to 0.1% VAF.

Products

VarTrace™ EGFR Assay

Detects mutations in exons 18, 19, 20, and 21 in FFPE and plasma samples

VarTrace™ AML Multi-gene Panel

Detects AML hotspot mutations in FLT3, DNMT3A, IDH1, IDH2, KIT, NPM1 genes

VarTrace™ BRAF Assay

Detects mutations in codons 596–601 in FFPE and plasma samples

VarMap™: NGS

Features

Affordable

Only 1 million reads for liquid biopsy

Ultrasensitive

Limit of detection down to 0.1% VAF

Quantitative

>95% VAF quantitation accuracy

Sample Flexibility

Compatible with several sample types

Sample Data

VarMap NGS Panels preferentially amplify variant molecules, resulting in a reduction of required sequencing reads by 60-fold compared to deep sequencing approaches.

Products

Varmap™ NSCLC NGS Panel

Detects over 1100 COSMIC mutations in AKT1, ALK, BRAF, DDR2, EGFR, ERBB2, KRAS, MAP2K1, MET, NRAS, PIK3CA, PTEN, ROS1, and TP53

VarMAP™ Melanoma NGS Panel

Detects over 370 COSMIC mutations in AKT1, AKT3, BRAF, KRAS, MAP2K1, MAP2K2, NRAS, and PIK3CA

Coming Soon:

Varmap™ pan-cancer NGS Panel

Detect over 6500 COSMIC mutations in over 60 genes

Customized Assays and Services

Features

Customizable

Automatically design custom BDA assays

Rapid Turnaround

qPCR, Sanger: 2-3 days

Rapid Prototype NGS Panel: 2 weeks

End-to-End NGS Panel Development: 6-12 weeks

Pan-Platform

qPCR, Sanger, and NGS

Ultrasensitive

Limit of detection down to 0.1% VAF

Sample Data

NGSure’s proprietary algorithm designs custom BDA assays with a limit of detection of 0.1% VAF, significantly expanding the range of mutations detected by NGS.

Products

NGSure qPCR/Sanger

Cost-effective customizable qPCR and Sanger-based BDA assays for the rapid and sensitive validation of variants identified by NGS.

Customized NGS design

Customizable NGS panel designs with 10-100 amplicons requiring less than 1 million reads. Two bespoke NGS design services available:

1) Rapid Prototype NGS Panel – custom panels designed and delivered in 2 weeks

2) End-to-End NGS Panel – custom panels designed and rigorously optimized by NuProbe’s expert team for delivery in 6-12 weeks