Limit of detection down to 1% VAF
Accurate results with as little as 3ng input DNA
Matched normal not required
Included software reports MSI-H, MSI-L/MSS
Results in less than two hours
Batch up to 47 samples per qPCR run
Compatible with tumor tissue or cell-free DNA (cfDNA)
Microsatellite Instability Markers Covered
BAT-25, BAT-26, NR-21, NR-24, and MONO-27
- MSI Determination
- The MSI status is based on the Ct values for each marker in the sample, by comparing with the GAPDH internal control to normalize the sample input. If the Ct value of marker is earlier than threshold, the marker is a positive. And based on the number of positive markers out of the 5 markers, we can call the sample MSI-H (>2/5); MSI-L(1/5) or MSS(0/5).
- MSI Software
- We provide an easy-to-use software to interpret MSI results and generate a report. Simply upload the qPCR data into the NuProbe MSI software, and the software gives sample results in a plate view map by color code. Users can then label the samples, view the result for each marker and qPCR curves, and export the MSI status report in excel format.
Step 1: DNA Extraction
DNA is extracted from clinical samples with commercial extraction kits such as cobas® cfDNA Sample Preparation Kit, QIAamp® DNA Blood Mini Kit, GeneRead® DNA FFPE Kit.
Step 2: qPCR BDA Reaction
NuProbe’s qPCR kit reagents and clinical samples are prepared for qPCR reactions. After running the reaction, Ct results are obtained.
1.5 hrs (15 min hands-on)
Step 3: Results Interpretation
The MSI status of each sample will be visualized and reported by the easy-to-use VarTrace MSI software.
2 min hands-on
|Technology||multi-blocker mBDA & qPCR|
|Kit size||48 specimens / kit|
|Biomarkers||Tube 1: BAT26, NR21, NR24|
|Tube 2: BAT25, Mono27|
|Tubes/wells per specimen||2|
|Turnaround time||qPCR only: 1h30min including 5min hands-on time|
|Limit of detection||≥ 10ng per reaction: 1%|
|< 10ng per reaction: 5%|
|Specificity||>98% at 1% VAF|
|Specimen type||Blood or FFPE|
|Specimen input||1.5-15 ng per reaction (3-30 ng per specimen) ideally 20ng (10 ng per tube)|
|Control||Positive (5%) and Negative|
|Amplicon length||[80, 120] bp|
|Storage condition||-20°C, up to 6 months|
|Software||Included software reports MSS, MSI-L, MSI-H based on N/5 abnormal|
Request Quote / Demo
Yan Helen Yan, Ph.D., senior research scientist at NuProbe. She obtained her Doctorate degree from Rice University and has over 7 years of research experience in nucleic acid diagnostics, genomics, qPCR and next-generation sequencing. Contact Helen for questions about the VarTrace MSI assay.